Recently, the team collaborated with Prof. Gao Mingyuan's project group from Soochow University, and have achieved significant progress in real-time tracking of various targeted free radicals and investigated their pathological development. Related findings are published in the Nature Communications journal, with SSIJRI as the first affiliation.
Precise and differential profiling of the dynamic correlations and pathophysiological implications of multiplex biological mediators with deep penetration and highly programmed precision remain critical challenges in clinics. Here we present an innovative strategy by tailoring a powerful multispectral optoacoustic tomography (MSOT) technique with a photon-upconverting nanoprobe (UCN) for simultaneous visualization of diversely endogenous redox biomarkers with excellent spatiotemporal resolution in living conditions. Upon incorporating two specific radicals-sensitive NIR cyanine fluorophores onto UCNs surface, such nanoprobes can orthogonally respond to disparate oxidative and nitrosative stimulation, and generate spectrally opposite optoacoustic signal variations, which thus achieves compelling superiorities for reversed ratiometric tracking of multiple radicals under dual independent wavelength channels, and significantly, for precise validating of their complex dynamics and correlations with redox-mediated pathophysiological procession in vivo.
Author information：Xiangzhao Ai, Zhimin Wang, Haolun Cheong, Yong Wang, Ruochong Zhang, Jun Lin, Yuanjin Zheng, Mingyuan Gao*, Bengang Xing
Multispectral optoacoustic imaging of dynamic redox correlation and pathophysiological progression utilizing upconversion nanoprobes
Nature Communications , Article number: 1087 (2019) , Pub Date : 2019-03-06 , DOI: 10.1038/s41467-019-09001-7